Synthesis, molecular docking study, and in vivo biological evaluation of pyrazolopyridines derived from monocarbonyl curcumin analogues as potential anti-inflammatory agents

Background: Pyrazolopyridines are heterocyclic compounds with nitrogen atoms in the ring, and they have been used as one of important pharmacophores drug design. Pyrazole derivatives synthesised applied pharmaceutical industry active drugs. The recent commercial success pyrazole COX-2 inhibitors has brought even more attention to significance this ring medicinal chemistry. Objective: This study aimed synthesise new anti-inflammatory candidates from pyrazolopyridine group. Method: Synthesis was carried out using Claisen-Schmidt reaction through condensation substituted benzaldehyde 4-piperidone produce mono-ketone curcumin analogues, which were then cyclised phenylhydrazine. results synthesis characterised FT-IR, 1H-NMR, MS. Docking analysis performed on 3LN1 protein MOE 2021.0901. Furthermore, an vivo test a plethysmometer. Results: obtained two compounds, docking showed that both these could interact receptor binding site. Conclusion: Compound 2 demonstrated good activities. strategy is preliminary stages identifying novel substances may be agents future.